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MBD1 (Clone 100B272.1) Antibody
Monoclonal antibody to detect MBD1 (a CpG-specific DNA demethylase) in Human samples
Product Overview
Product Name: MBD1 (Clone 100B272.1) Antibody
Antibody Target: MBD1
Alternative Name: Methyl-CpG-Binding Domain 1
Host: Mouse
Antibody Type: Monoclonal
Isotype: Mouse IgG1
Immunogen: Human MBD1 amino acids 391-405
Accession#: Q9UIS9
Gene ID: 4152
Appearance: Colorless liquid
Concentration: N/A
Formulation: 50 µg in 100 µl PBS containing 0.2% gelatin and 0.05% sodium azide.
Purification: Affinity chromatography purified via peptide column (protein G)
Species Reactivity: Human
Application: Western blot
Positive Control: HeLa cell lysate
Application & Usage: Western blot analysis (2-4 µg/ml). However, the optimal conditions should be determined individually.
Storage Temp.: -20 °C
Shipping: Gel Pack
Background Descriptions: DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD).The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) co-localize with foci of heavily methylated satellite DNA and mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA either in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 has homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans. |