|
IL-17A Antibody
Polyclonal antibody to detect IL-17A in human samples
Product Overview
Product Name: IL-17A Antibody
Antibody Target: IL-17A
Alternative Name: CTLA-8, IL-17, IL-17A, Cytotoxic T-lymphocyte associated antigen 8
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: E.coli derived Recombinant Human IL-17A
Accession#: Q16552
Gene ID: 3605
Appearance: Lyophilized powder
Concentration: N/A
Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Purification: Affinity chromatography
Species Reactivity: Human
Application: Western blot, ELISA, Neutralization, Immunohistochemistry
Positive Control: ELISA: Recombinant hIL-17A (Cat # 4176). IHC: Human breast invasive ductal carcinoma tissue section
Application & Usage: 1) WB: Use 0.1-0.2 µg/ml. The detection limit for recombinant human IL-17A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
2) ELISA: Use 0.5 - 2.0 μg/ml (100 μl/well antibody solution)
3) Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-17A (50.0 ng/ml), a concentration of 0.9-1.3 μg/ml of this antibody is required.
4) Immunohistochemistry: 0.25 µg/ml with an overnight incubation at 4°C.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: The originally described IL-17 protein, now known as IL-17A, is a homodimer of two 136 amino acid chains, secreted by activated T-cells that act on stromal cells to induce production of proinflammatory and hematopoietic bioactive molecules. Today, IL-17 represents a family of structurally-related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. The six known members of this family, IL-17A through IL-17F, are secreted as homodimers. IL-17A exhibits cross-species bioactivity between human and murine cells.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans. |