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IL-7 Antibody
Polyclonal antibody to detect IL-7 in human samples
Product Overview
Product Name: IL-7 Antibody
Antibody Target: IL-7
Alternative Name: Lymphopoietin 1(LP-1), pre-B cell factor
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: E.coli derived Recombinant Human IL-7
Accession#: P13232
Gene ID: 3574
Appearance: Lyophilized powder
Concentration: N/A
Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Purification: Affinity chromatography
Species Reactivity: Human
Application: Western blot, ELISA, Neutralization
Positive Control: ELISA: Recombinant hIL-7 (Cat # 4146)
Application & Usage: 1) WB: Use 0.1-0.2 µg/ml. The detection limit for recombinant human IL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
2) ELISA: Use 0.5 - 2.0 μg/ml (100 μl/well antibody solution)
3) Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-7 (1.0 ng/ml), a concentration of 0.04 - 0.06 µg/ml of this antibody is required.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: IL-7 is a hematopoietic growth factor which affects primarily early B and T cells. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as Con A and IL-2. Human and murine IL-7 is cross-species reactive.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans. |