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Sushi Antibody
Purified Sushi polyclonal Antibody
Product Overview
Product Name: Sushi Antibody
Antibody Target: Sushi 3
Alternative Name: Factor C Sushi 3 antibody, Sushi 3 antibody
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Sushi domain in from E. coli
Accession#: N/A
Gene ID: N/A
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 100 µg (0.5 mg/ml) polyclonal antibody in PBS pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
Purification: Affinity purified
Species Reactivity: All
Application: Western blot
Positive Control: Recombinant Sushi protein
Application & Usage: Western Blot analysis (1-4 µg/ml).
Storage Temp.: -20 °C
Shipping: Gel Pack
Background Descriptions: Sushi Peptide S3 is a trimer of one of the high endotoxin-binding domains, Sushi 3 (or S3) within Factor C, a lipopolysaccharide (LPS)-sensitive serine protease of the horseshoe crab (Limulus Polyphemus). S3 display detergent-like properties in disrupting LPS aggregates, with specificity for palmitoyl-oleoyl-phosphatidylglycerol (POPG) resulting from electrostatic and hydrophobic forces between the peptides and the bacterial lipids. The unsaturated nature of POPG confers fluidity and enhances insertion of the peptides into the lipid bilayer, causing maximal disruption of the bacterial membrane. In short, peptide S3 can bind to lipopolysaccharide (LPS) and inhibit the growth of Gram-negative bacteria without affecting mammalian cells. It has been shown that endotoxin activates Factor C based catalytic coagulation cascade resulting in the gelation of Limulus blood. This process is the basis of Limulus Amebocyte Lysate (LAL) endotoxin detection method.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans. |