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DNMT3a Antibody
Polyclonal Antibody to detect DNMT3a in human, mouse and rat samples
Product Overview
Product Name: DNMT3a Antibody
Antibody Target: DNMT3a
Alternative Name: DNA Methyltransferase 3a, DNMT3A
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit Ig
Immunogen: Synthetic peptide at near N-terminal
Accession#: Q9Y6K1
Gene ID: 1788
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 100 µg (0.5 mg/ml) of antibody in PBS, 0.01 % BSA, 0.01 % thimerosal, and 50 % glycerol, pH 7.2
Purification: Affinity purified
Species Reactivity: Human, mouse, rat
Application: Western blot
Positive Control: Western blot: Jurket cell lysate
Application & Usage: Western blot: 1:200
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting, and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, three families of mammalian DNA methyltransferase genes have been identified which include DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. DNMT2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The DNMT3 family members, DNMT3a and DNMT3b, are strongly expressed in embryonic stem (ES) cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of DNMT3b gene occur in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans. |