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Enteropeptidase/Enterokinase Cleavage Kit
Product Overview
Assay Kit Name:
Enteropeptidase/Enterokinase Cleavage Kit
Kit Summary:
Sufficient for cleaving at least 5 mg of target protein
Sample Type:
N/A
Features & Benefits:
• Efficiently removes tags from recombinant fusion proteins containing accessible Enteropeptidase-specific recognition sequence.
• Includes cleavage control protein (hPRL-Trx)
Kit Components:
• Enteropeptidase Cleavage Buffer
• Human Enteropeptidase (100 U)
• Cleavage Control Protein (hPRL-Trx)
Description:
Enteropeptidase (Enterokinase, EC 3.4.21.9) is a serine protease involved in activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence ‘DDDDK’ and cleaves after the lysine residue (K). The high specific activity of Enteropeptidase has been utilized in cleaving a variety of native or fusion proteins containing the above recognition motif. Biovision’s Enteropeptidase Cleavage Kit contains highly active light chain fragment of human Enteropeptidase. Our pure enzyme is an excellent tool to obtain a wild type protein sequence from a fusion protein, containing Enteropeptidase recognition sequence (DDDDK). This Enteropeptidase cleavage kit is sufficient for cleaving at least 5 mg of target protein. The residual enteropeptidase left in the reaction mix will not interfere with most of the downstream applications. Following cleavage of the target protein, Enteropeptidase can be removed using BioVision Hi-Bind Ni-QR Agarose beads.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans. |