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IGF-1 (human) ELISA Kit
Product Overview
Assay Kit Name:
IGF-1 (human) ELISA Kit
Kit Summary:
• Detection method - Absorbance (450 nm)
• Species reactivity - human
• Applications - Quantitative protein detection, establishing normal range, validation of antibody array results
Sample Type:
• Serum & plasma
• Cell culture supernatants
• Urine
Features & Benefits:
• Easy, convenient and time-saving method to assay IGF-1.
• The minimum detectable concentration of IGF-1 is less than 0.1 ng/ml.
• This ELISA kit only can detect a free form of human IGF-I & shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF.
Kit Components:
• IGF-I Microplate (Item A) coated with anti-human IGF-I, 96 wells
• Wash Buffer Concentrate (20x) (Item B)
• Standards (Item C) (recombinant human IGF-I)
• Assay Diluent C (Item L) diluent buffer, 2 bottles
• Detection Antibody IGF-I (Item F), biotinylated anti-human IGF-I (each vial enough for half plate)
• HRP-Streptavidin Concentrate (Item G), 120x concentrated
• TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution
• Stop Solution (Item I), 0.2 M sulfuric acid
Description:
BioVision’s human IGF-I ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IGF-I. This assay employs an antibody specific for human IGF-I coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF-I present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IGF-I antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF-I bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit only can detect a free form of human IGF-I & shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of IGF-I is typically less than 0.1 ng/ml. Detection Range: 0.1 ng/ml - 30 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans. |