|
Resistin (human/mouse/rat) EIA Kit
Product Overview
Assay Kit Name:
Resistin (human/mouse/rat) EIA Kit
Kit Summary:
• Detection method - Detection method- Absorbance (450 nm)
• Species reactivity - Species reactivity- human, mouse, rat
• Applications - Application- Quantitative protein detection, establishing normal range, validation of antibody array results
Sample Type:
• Serum & plasma
• Culture supernatants & cell lysates
Features & Benefits:
• Easy, convenient and time-saving method to assay Resistin.
• The minimum detectable concentration of Resistin is less than 446 pg/ml.
• This EIA kit detects Resistin (90 aa). No other active isoforms have been reported. This kit shows no cross-reactivity with the following cytokines tested: e.g., Ghrelin, Nesfatin, Angiotensin II, NPY and APC.
Kit Components:
• Resistin Microplate (Item A) coated with secondary antibody, 96 wells
• Wash Buffer Concentrate (20x) (Item B)
• Standard Resistin Peptide (Item C) (10 μl/vial)
• Anti-Resistin polyclonal antibody (item N) (5 μl/vial)
• Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative)
• Assay Diluent B (Item E) for Standard/Sample (cell culture medium or other sample) diluent. (5x concentrated)
• Biotinylated Resistin peptide (Item F), (20 μl/vial)
• HRP-Streptavidin Concentrate (Item G), 160x concentrated
• Positive Control (Item M) (100 μl)
• TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution
• Stop Solution (Item I), 0.2 M sulfuric acid
Description:
BioVision’s Resistin [XCP-1 (CEBPE regulated myeloid-specific secreted cysteine-rich protein precursor 1), FIZZ3 (found in inflammatory zone 3), or ADSF (adipocyte-specific secretory factor)] Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Resistin peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti-Resistin antibody, both biotinylated Resistin peptide and peptide standard or targeted peptide in samples interacts competitively with the Resistin antibody. Uncompeted (bound) biotinylated Resistin peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of Resistin peptide in the standard or samples. This is due to the competitive binding to Resistin antibody between biotinylated Resistin peptide and peptides in standard or samples. A standard curve of known concentration of Resistin peptide can be established and the concentration of Resistin peptide in the samples can be calculated accordingly. The minimum detectable concentration of Resistin is 446 pg/ml. The detection range for the kit is 0.1-1,000 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<15%. This EIA kit detects Resistin (90 aa). No other active isoforms have been reported. This kit shows no cross-reactivity with the following cytokines tested: e.g., Ghrelin, Nesfatin, Angiotensin II, NPY and APC.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans. |