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Irisin Competitive ELISA Kit
Product Overview
Assay Kit Name:
Irisin Competitive ELISA Kit
Kit Summary:
• Detection method - Absorbance (450 nm)
• Species reactivity - Human, mouse, rat, monkey
• Applications - Quantitative ELISA
Sample Type:
• Serum & plasma
• Cell culture supernatants
Features & Benefits:
• Easy, convenient and time-saving method to assay Irisin.
• The minimum detectable concentration of Irisin is 1 ng/ml.
• This ELISA is specific for the measurement of natural and recombinant irisin in human samples. It should also work in mouse, rat and monkey biological samples. It does not cross-react with FNDC4, human adiponectin, human Nampt, human RBP4, human clusterin, human leptin, human vaspin, human GPX3, human resistin, human ACE2, human lipocalin-2, human ANGPTL3, human ANGPTL6, human DNER, human DLK1, human calreticulin, human IL-33, mouse Nampt, mouse clusterin, mouse vaspin, mouse resistin.
Kit Components:
• Plate coated with Irisin Recombinant Protein (12 x 8-well strips)
• Wash Buffer 10X (50 ml)
• Diluent 5X (50 ml)
• Detection Antibody (12 ml)
• Detector 100X (HRP Conjugated anti-rabbit IgG) (150 μl)
• Irisin Standard (lyophilized) (5 μg)
• Irisin QC sample (lyophilized)
• TMB Substrate Solution (12 ml)
• Stop Solution (12 ml)
• Plate sealers (plastic film)
Description:
BioVision’s Irisin (human) Competitive ELISA kit is to be used for the in vitro quantitative determination of human irisin in cell culture supernatants, serum and plasma. It should also work for the in vitro quantitative determination of irisin in mouse, rat and monkey biological samples. A polyclonal antibody recognizing native irisin reacts with a series of predetermined recombinant irisin standard proteins or samples under competition in the irisin-coated plate. Their relative reactivity is plotted with that of the standard proteins. Detection limit: 1 ng/ml. Note: The Limit of detection was measured by adding two standard deviations to the mean value of 50 zero standard. Assay Range: 0.001 ug/ml – 5 ug/ml. Irisin levels range in human plasma and serum from 0.2 to > 2 ug /ml.
Storage Conditions:
4°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans. |