|
Nesfatin (human/mouse/rat) EIA Kit
Product Overview
Assay Kit Name:
Nesfatin (human/mouse/rat) EIA Kit
Kit Summary:
Detection method- Absorbance (450 nm)
• Species reactivity- human, mouse, rat
• Applications- Quantitative protein detection, establishing normal range, validation of antibody array results.
Sample Type:
• Serum & plasma
• Cell culture media etc.
Features & Benefits:
• Easy, convenient and time-saving method to assay Nesfatin.
• The minimum detectable concentration of Nesfatin is typically less than 147 pg/ml.
• This EIA kit shows no cross-reactivity with the following cytokines tested: e.g., Ghrelin, Angiotensin II, NPY and APC.
Kit Components:
• Nesfatin Microplate (Item A): 96 wells (12 strips x 8 wells) coated with secondary antibody.
• Wash Buffer Concentrate (20x) (Item B): 25 ml.
• Standard Nesfatin peptide (Item C): 2 vials, 10 ul/vial.
• Anti- Nesfatin polyclonal antibody (Item N): 2 vials, 5 ul/vial.
• Assay Diluent A (Item D): 30 ml, contains 0.09% sodium azide as preservative. Diluent for standards and serum or plasma samples.
• Assay Diluent B (Item E): 15 ml, 5X concentrated buffer. Diluent for standards and cell culture media or other sample types.
• Biotinylated Nesfatin peptide (Item F): 2 vials, 20 ul/vial.
• HRP-Streptavidin concentrate (Item G): 600 µl 200x concentrated HRP-conjugated Streptavidin.
• Positive control (Item M): 1 vial, 100 ul.
• TMB One-Step Substrate Reagent (Item H): 12 ml of 3, 3’, 5, 5’- tetramethylbenzidine (TMB) in buffered solution.
• Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid.
Description:
BioVision’s Nesfatin Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Nesfatin peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- Nesfatin antibody, both biotinylated Nesfatin peptide and peptide standard or targeted peptide in samples interacts competitively with the Nesfatin antibody. Uncompeted (bound) biotinylated Nesfatin peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of Nesfatin peptide in the standard or samples. This is due to the competitive binding to Nesfatin antibody between biotinylated Nesfatin peptide and peptides in standard or samples. A standard curve of known concentration of Nesfatin peptide can be established and the concentration of Nesfatin peptide in the samples can be calculated accordingly. EIA-NES-1 detects 420aa Nesfatin protein. No other active isoforms have been reported. The minimum detectable concentration of Nesfatin is 147 pg/ml or 3.09 pM. The detection range for the kit is 0.1-1,000 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans. |