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TACE (human) ELISA Kit
Product Overview
Assay Kit Name:
TACE (human) ELISA Kit
Kit Summary:
Detection method- Absorbance (450 nm)
• Species reactivity- Human
• Applications- Quantitative protein detection, establishing normal range, validation of antibody array results.
Sample Type:
• Serum & plasma
• Cell culture supernatants
• Urine
Features & Benefits:
• Easy, convenient and time-saving method to assay for human TACE.
• The minimum detectable dose of TACE is typically less than 70 pg/ml.
• This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g. human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-γ, IGFBP-2, IGF-BP-3, IGF-BP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF,SDF-1alpha, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF.
Kit Components:
• TACE Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human TACE.
• Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.
• Standards (Item C): 2 vials, recombinant human TACE.
• Assay Diluent C (Item L): 30 ml of diluent buffer. For Standard/Sample (serum/plasma/ cell culture medium/urine) diluent.
• Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For detection antibody & HRP-streptavidin diluent.
• Detection Antibody TACE (Item F): 2 vial of biotinylated anti human TACE (each vial is enough to assay half microplate).
• HRP-Streptavidin Concentrate (Item G): 200 µl of 200x concentrated HRP-conjugated streptavidin.
• TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’tetramethylbenzidine (TMB) in buffered solution.
• Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid.
Description:
BioVision’s Human TACE (TNF-alpha converting enzyme) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TACE. This assay employs an antibody specific for human TACE coated on a 96-well plate. Standards and samples are pipetted into the wells and TACE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TACE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TACE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of TACE is typically less than 70 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans. |