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BMP-4 (human) ELISA Kit (For Lysates)
Product Overview
Assay Kit Name:
BMP-4 (human) ELISA Kit (For Lysates)
Kit Summary:
Detection method- Absorbance (450 nm)
• Species reactivity- Human
Sample Type:
• Cell lysate & tissue lysate
Features & Benefits:
• Easy, convenient and time-saving method to assay for human BMP-4 in cells & tissues.
• The minimum detectable dose of BMP-4 is typically less than 15 pg/ml.
• This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, BMP-6, BMP-7, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF.
Kit Components:
• BMP-4 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human BMP-4.
• Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.
• Standards (Item C): 2 vials, recombinant human BMP-4.
• Sample Diluent Buffer (Item D): 10 ml of 5x concentrated buffer. For Standard/Sample (cell lysate/tissue lysate) diluent.
• Assay Diluent (Item E): 15 ml of 5x concentrated buffer. For Detection Antibody (Item F) & HRP-Streptavidin Concentrate (Item G) diluent.
• Detection Antibody BMP-4 (Item F): 2 vial of biotinylated anti human BMP-4 (each vial is enough to assay half microplate).
• HRP-Streptavidin Concentrate (Item G): 200 µl of 80x concentrated HRP-conjugated streptavidin.
• TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’tetramethylbenzidine (TMB) in buffered solution.
• Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid.
• Cell lysate buffer (Item J): 5 ml 2X cell lysate buffer.
Description:
BioVision’s Human BMP-4 (Bone morphogenetic proteins-4) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BMP-4. This assay employs an antibody specific for human BMP-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BMP-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of BMP-4 is typically less than 15 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans. |