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IL-17F (human) ELISA Kit
Product Overview
Assay Kit Name:
IL-17F (human) ELISA Kit
Kit Summary:
Detection method- Absorbance (450 nm)
• Species reactivity- Human
Sample Type:
• Serum & plasma
• Cell culture supernatants
• Urine
Features & Benefits:
• Easy, convenient and time-saving method to assay for human IL-17.
• The minimum detectable dose of IL-17 is typically less than 80 pg/ml.
• This ELISA kit is specific for the measurement of human IL-17F. The kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-17C, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1alpha, MIP-1β, MIP-1, MMP-1, -2, -3, -10, PARC, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-alpha, TNF-β, TPO, VEGF).
Kit Components:
• IL-17F Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human IL-17F.
• Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.
• Standards (Item C): 2 vials, recombinant human IL-17F.
• Assay Diluent C (Item L): 30 ml of diluent buffer. For Standard/Sample (serum/plasma/cell culture medium/urine) diluent.
• Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For detection Antibody & HRP-Streptavidin diluent.
• Detection Antibody IL-17F (Item F): 2 vial of biotinylated anti-human IL-17F (each vial is enough to assay half microplate)
• HRP-Streptavidin Concentrate (Item G): 200 µl of 200x concentrated HRP-conjugated streptavidin.
• TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’tetramethylbenzidine (TMB) in buffered solution.
• Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid.
Description:
BioVision’s Human IL-17F ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-17F. This assay employs an antibody specific for human IL-17F coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17F present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-17F antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17F bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of IL-17F is typically less than 20 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-17C, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1alpha, MIP-1β, MIP-1, MMP-1, -2, -3, -10, PARC, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-alpha, TNF-β, TPO, VEGF).
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans. |