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HDAC1 Immunoprecipitation (IP) & Activity Assay Kit
Product Overview
Assay Kit Name:
HDAC1 Immunoprecipitation (IP) & Activity Assay Kit
Kit Summary:
Detection method - Fluorescence (Ex/Em=380/500 nm)
• Applications:
- Immunoprecipitation of HDAC1 complex from cell, nuclear, or tissue extract(s)
- Measurement of HDAC1 activity of immunoprecipitated complex/purified enzyme
- Screening for activators or inhibitors of HDAC1
Sample Type:
• Human, Mouse or Rat
• Cell Lysate and Nuclear Extract
• Tissue Extract
Features & Benefits:
• Simple, fast and convenient.
• Only kit on the market for both IP as well as measuring activity of HDAC1
Kit Components:
• HDAC Assay Buffer
• Cell Lysis Buffer
• HDAC Substrate
• Developer
• AFC (7-amino-4-trifluoromethyl coumarin) Standard (1 mM)
• Rabbit HDAC1 Antibody
• Rabbit IgG (Control Antibody)
• Protein-A/G Sepharose Beads (50% slurry in 20% ethanol/H2O)
• Positive Control (Jurkat Cell Lysate) Lyophilized
Description:
Histone deacetylases (HDACs) play a central role in controlling cell cycle regulation, cell differentiation, and tissue development. HDAC1 is ubiquitously expressed and plays a role in cellular senescence, cardiomyocyte differentiation and hypertrophy, aging of the liver, myelination, and adult neurogenesis to name a few functions. The HDAC1 IP & Activity Assay Kit provides an antibody-based method to specifically immunoprecipitate the HDAC1 complex from cells & tissues and to measure HDAC1 activity fluorometrically. HDAC1 is immunoprecipitated from cell, nuclear or tissue extract(s) using HDAC1 antibody followed by capturing the complex on protein-A/G beads. The immunoprecipitated complex is incubated with the HDAC substrate. Only the deacetylated substrate is cleaved by the Developer to produce a fluorophore, which can be easily analyzed using a fluorescence plate reader.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans. |