Citrate Synthase Activity Colorimetric Assay Kit

Citrate Synthase Activity Colorimetric Assay Kit
Product Overview
Assay Kit Name:
Citrate Synthase Activity Colorimetric Assay Kit
Kit Summary:
• Detection method - Absorbance (412 nm)
• Species reactivity - N/A
• Applications:
-Measurement of citrate synthase activity in various tissues/cells
- Analysis of intact mitochondria
Sample Type:
• Animal tissues: liver, heart, kidney, etc.
• Cell culture: Adherent or suspension cells
• Purified mitochondria
Features & Benefits:
• Simple, rapid & convenient
• The assay can detect Citrate Synthase Activity less than 1 mU in a variety of samples
Kit Components:
• CS Assay Buffer
• CS Substrate Mix (Lyophilized)
• CS Developer (Lyophilized)
• GSH Standard (reduced) (Lyophilized)
• CS Positive Control (Lyophilized)
Description:
Citrate Synthase (EC 2.3.3.1) is a key enzyme that is present in all living organisms. It catalyzes the conversion of acetyl-CoA and oxaloacetate into citrate and serves as a marker for intact mitochondria. A recent study showed that increased activity of mitochondrial Citrate Synthase is directly associated with metabolic and endocrine abnormalities such as obesity. In BioVision’s Citrate Synthase Activity Assay Kit, Citrate Synthase reacts with substrate mix to form an intermediate, which subsequently reacts with developer to generate the colored product. The rate of color development is proportional to the enzyme activity. The assay is simple, rapid and can detect Citrate Synthase activity less than 1 mU in a variety of samples.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.