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Screen Quest� Colorimetric Glucose Uptake Assay Kit
Cat # 36504
Storage F/D/L (see storage codes below)
Unit Size 500 Tests
Ex (nm) 575
Glucose transport systems are responsible for transporting glucose across cell membranes. Measuring uptake of 2-deoxyglucose (2-DG), a glucose analog, in tissues and cells is widely accepted as a reliable method to estimate the amount of glucose uptake and to investigate the regulation of glucose metabolism and mechanism of insulin resistance. The 2-DG uptake is commonly determined by using non-metabolized 2-DG labeled with tritium or C14. However, routine use of a radiolabelled probe is costly and requires a tedious special handling procedure. AAT Bioquest抯 Screen Quest� Colorimetric Glucose Uptake Assay Kit provides a sensitive and non-radioactive assay in tissues or cultured cells. In this assay 2-DG is taken up by glucose transporters, and metabolized to 2-DG-6-phosphate (2-DG6P). The non-metabolizable 2-DG6P accumulates in the cells, and is proportional to glucose uptake by cells. The accumulated 2-DG6P is enzymatically oxidized and generates NADPH, which is specifically monitored by a chromogenic NADPH sensor. The signal can be read by a absorption microplate reader by reading the OD ratio at wavelength 570 nm to 610 nm.
Detailed Information: Protocol References MSDS
Related Products: Glucose Transporters
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
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