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Progranulin (rat) ELISA Kit
Product Overview
Assay Kit Name:
Progranulin (rat) ELISA Kit
Kit Summary:
• Detection method- Absorbance (450 nm)
• Species reactivity- Rat
Sample Type:
• Serum
• Cell culture supernatants
Features & Benefits:
• The lowest level of progranulin that can be detected by this assay is 40 pg/ml
• The Assay range is 0.063 ng/ml – 4 ng/ml
• This ELISA is specific for the measurement of natural and recombinant rat progranulin. It does not cross-react with human progranulin, human granulin C, human granulin F, rat RBP4, rat adiponectin, rat Nampt, rat ANGPTL4, rat lipocalin-2, rat resistin, mouse RBP4, mouse adiponectin, human RBP4, human adiponectin.
Kit Components:
• 1 plate coated with rat progranulin Antibody (12 x 8-well strips)
• 1 bottle Wash Buffer 10X (50 ml)
• 1 bottle Diluent 5X (50 ml)
• 1 bottle Detection Antibody (12 ml)
• 1 vial Detector 100X (HRP Labeled Streptavidin) (150 μl)
• 1 vial human progranulin Standard (lyophilized) (8 ng)
• 1 vial human progranulin QC sample (lyophilized)
• 1 bottle TMB Substrate Solution (12 ml)
• 1 bottle Stop Solution (12 ml)
• 3 plate sealers (plastic film)
Description:
BioVision’s Progranulin (rat) ELISA kit is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human progranulin in biological fluids. A polyclonal antibody specific for progranulin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, progranulin is recognized by the addition of a biotinylated polyclonal antibody specific for progranulin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of progranulin in the samples.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans. |