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ADP Colorimetric Assay Kit II
Product Overview
Assay Kit Name:
ADP Colorimetric Assay Kit II
Kit Summary:
• Detection method - Absorbance (450 nm)
• Application - The kit can detect less than 20 μM of ADP in samples.
Sample Type:
• Animal tissues - Liver, Muscle, Heart etc.
• Cell culture - Adherent or suspension cells
Features & Benefits:
• Simple procedure; takes ~ 20 minutes
• Convenient and sensitive
• Kit contains all necessary reagents for accurate measurement of ADP levels
Kit Components:
• ADP Assay Buffer
• ADP Enzyme Mix (Lyophilized)
• Developer (Lyophilized)
• Probe (Lyophilized)
• ADP Standard (Lyophilized)
Description:
ADP is a product of ATP dephosphorylation and it can be rephosphorylated to ATP. Dephosphorylation and rephosphorylation occur via various phosphorylases and kinases. ADP is stored in platelets and can be released to interact with variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories. BioVision ADP Assay kit II is suitable for measuring ADP levels in samples that contain reducing substances, which may interfere with oxidase-based assays. In this assay, ADP in the presence of ADP Enzyme Mix is converted to an intermediate, which reduces a colorless Probe to a colored product with strong absorbance at 450 nm. ADP Assay Kit II is simple, fast and high-throughput ready. It can detect less than 20 μM of ADP in samples.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans. |