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Chloroethyl Ubiquitin (HA-tag)
Description:
Chloroethyl Ubiquitin (HA-tag)
Trade Name:
Upstate (Millipore)
Qty/Pk:
25 µg
Background Information:
The ubiquitin cascade in its many forms includes a large family of cysteine proteases known as deubiquitinylating enzymes (DUBs). Many members are assigned to this enzyme class by sequence similarity but with little evidence for specific biological activity. HA-tagged ubiquitin molecules derivatised by chemical ligation at the C-terminus with thiol-reactive functional groups can be used as potent, irreversible inhibitors of DUBs through their covalent modification of the active site and, consequently, can act as specific probes for enzymes with DUB activity. Such probes have been utilized for the identification of DUBs and associated components from cell lysates by proteomic methods, as well as rapid demonstration of enzymatic activity for gene products whose functions were inferred from primary structure. The nature of the electrophilic group used as the C-terminal derivative, for example vinyl sulfone (-VS), vinyl methylester (-VME), 2-bromoethyl (-Br) or 2-chloroethyl (-Cl), may determine the subset of DUB enzymes detected.
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Key Applications:
Protein Binding
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Molecular Weight:
10 kDa
Physical Form:
Frozen solution
Brand Family:
Upstate
Source:
Synthetic peptide
Presentation:
Lyophilized;reconstitute with aqueous buffer up to 5 mg/mL (~500 µM)
Format:
Purified
Storage Conditions:
After opening, prepare aliquots and store at -80ºC. Avoid freeze/thaw cycles. After reconstitution, prepare aliquots, store at -80ºC and use within 1 month. Stable for at least 6 months after receipt when stored at -80ºC.
Product Name:
Chloroethyl Ubiquitin (HA-tag)
Purity:
>95% by HPLC
Purification Method:
HPLC |