p300(catalytic domain)human recombinant

p300 (catalytic domain) human recombinant
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PRODUCT FAMILY INFORMATION
Recombinant Histone Proteins, Enzymes, Peptides & Inhibitors
Purified recombinant histones, native nucleosomes, highly active histone-modifying enzymes, and broad range of modified peptide substrates and inhibitors.
Perform intricate in vitro manipulations of histones, nucleosomes, and chromatin with our portfolio of purified recombinant histones, native nucleosomes, highly active histone-modifying enzymes, and broad range of modified peptide substrates.
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Description:
p300 (catalytic domain) human recombinant
Trade Name:
Upstate (Millipore)
Qty/Pk:
100 µg
Product Overview:
Human recombinant p300 (catalytic domain) expressed in E. Coli
Background Information:
Although originally termed histone acetyltransferases (HATs) for their lysine acetylation activity on histone N-terminal tails, p300 and its paralogue, CBP, have been shown to acetylate a variety of non-histone proteins including p53, DNA polymerase ß and nuclear import factors. p300/CBP acetylations play regulatory roles in transcription, DNA repair and replication, the cell cycle, p53 turnover, and nuclear import. In addition to functions which overlap with those of CBP, p300 has a number of unique functions, including a role in the induction of p21Waf1/Cip1 cell cycle inhibitor.
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Applications:
Human recombinant p300 (catalytic domain) expressed in E. Coli.
Key Applications:
Enzyme Assay
Application Notes:
Acetyltransferase assays
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Species:
Human
Molecular Weight:
~52 kDa
Brand Family:
Upstate
Source:
Catalytic domain of huma p300
Presentation:
In liquid containing 50 mM Tris/HCl, pH 8.0, 0.1 mM EDTA, 10% glycerol
Storage Conditions:
Stable for 12 months at -80ºC from date of receipt. Avoid multiple freeze/thaw cycles.
Product Name:
p300 (catalytic domain) human recombinant
Physical Form:
Frozen solution
Format:
Purified
Host:
E. coli
Specific Activity:
>250 pmol/min/µg assayed as production of CoA-SH from AcCoA in the presence of a peptide comprising human p53 residues 368-386. CoA-SH is determined colorimetrically by reaction with DTNB (5,5′-Dithiobis (2-nitrobenzoic acid).
Purity:
Purity >75% by SDS-PAGE