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Histone H2B human recombinant
Description:
Histone H2B human recombinant
Trade Name:
Upstate (Millipore)
Qty/Pk:
50 µg
Product Overview:
Human recombinant H2B expressed in E. Coli
Background Information:
Histone H2B is one of four core histones that form an octameric structure required for the packaging of DNA into nucleosomes, the most basic level of chromatin arrangement. Histones undergo a number of post-translational modifications (PTM) at their N- and C-terminal tails which can influence chromatin structure and stability and consequently, transcription, and DNA replication and repair. The most widely studied of these modifications are methylation, phosphorylation, and acetylation which may produce different effects on gene regulation depending on the number and pattern of modifications. Histone H2B undergoes acetylation at lysines 5, 15, and 20. It is also phosphorylated in apoptotic cells during DNA fragmentation, possibly in a caspase-dependent manner.
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Applications:
Human recombinant H2B expressed in E. Coli.
Key Applications:
Kinase Assay
DNA-binding Proteins
Western Blotting
Application Notes:
Chromatin assembly, Western blot (control), Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Species:
Human
Molecular Weight:
~17 kDa
Brand Family:
Upstate
Presentation:
Liquid in Dulbecco's PBS
Storage Conditions:
Stable for 12 months at -80ºC from date of receipt. Avoid multiple freeze/thaw cycles.
Product Name:
Histone H2B human recombinant
Physical Form:
Frozen solution
Format:
Purified
Host:
E. coli
Purity:
Purity >95% by SDS-PAGE |