|
RIPAb+™ STAU1 (Staufen 1) - RIP Validated Antibody and Primer Set
Close
REFERENCES
Molecular genetic analysis of malignant melanomas for aberrations of the WNT signaling pathway genes CTNNB1, APC, ICAT and BTRC.
Reifenberger, Julia, et al. (2002) Int. J. Cancer, 100: 549-56 (2002)
Species Reactivity Key Applications Host Format Antibody Type
H WB, IP, RIP Rat null Polyclonal Antibody
Description:
RIPAb+™ STAU1 (Staufen 1)
Promotional Text:
Special Offer on Antibodies! Click Here!
Product Overview:
RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
STAU1 (Staufen 1) is a RNA-binding protein implicated in the transport and/or localization of mRNA via the microtubule network to the rough endoplasmic reticulum.
View All »
Molecular Weight:
~63 kDa observed. Uncharacterized bands appear at ~22 kDa and ~53 kDa in some lysates.
Epitope:
a.a. 2-51
Immunogen:
Synthetic peptide from amino acids 2-51 of human STAU 1.
Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with human. Other species have not yet been tested.
Application Notes:
Immunoprecipitation from RIP lysate:
Representative lot data.
RIP lysate from HeLa cells (~2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal rabbit IgG, (Cat. #PP64B), or 5 µg of Anti-STAU1 (Staufen 1) antibody (Cat. # CS204405). Ten percent of the precipitated proteins (lane 1: rabbit IgG, lane 2: STAU1) were resolved by electrophoresis, transferred to nitrocellulose and probed with Anti-STAU1 (Staufen 1) antibody (Cat. # CS204405, 1:1000). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00232) .
Arrow indicates STAU1. (Figure 2).
Western Blot Analysis:
Representative lot data.
HepG2 cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-STAU1 antibody (Cat. # CS204405, 1:1000 dilution). Proteins were visualized using a Goat Anti-Rabbit IgG conjugated to HRP and a chemiluminescence detection system.
Arrow indicates STAU1 (~63 kDa). (Figure 3).
Note: Uncharacterized bands appear at ~22 kDa and ~53 kDa in
View All »
Control:
Includes negative control normal rabbit IgG antibody and control primers specific for the human ARF1 mRNA.
Quality Assurance:
RNA Binding Protein Immunoprecipitation:
RIP Lysate prepared from HeLa cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal Rabbit IgGor 5 µg of Anti-STAU1 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of STAU1-associated RNA was verified by qPCR using RIP Primers ARF1, (Figure 1).
Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) |