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Anti-phospho-SPAK Antibody (Ser373) / phospho-OSR1 Antibody (Ser325)
Description:
Anti-phospho-SPAK Antibody (Ser373) / phospho-OSR1 Antibody (Ser325)
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Specificity:
This antibody recognizes SPAK phosphorlyated at Ser373 and OSR1 phosphorlyated at Ser325.
Molecular Weight:
~60 kDa observed
Epitope:
SPAK phosphorlyated at Ser373 and OSR1 phosphorlyated at Ser325.
Immunogen:
KLH-conjugated linear peptide corresponding to human SPAK and OSR1 phosphorylated at Ser375 and Ser325, respectively.
Modifications:
Phosphorylation
Background Information:
As members of the (sterile 20)-like (STE20) kinase family, the oxidative-stress-responsive kinase 1 (OSR1) and the SPS1-related proline/alanine-rich kinase (SPAK) serve as key enzymes in a signaling cascade, which mediate the activity of Na+/K+/2Cl- cotransporters (NKCCs) in response to osmotic stress. Both kinases have a conserved carboxy-terminal (CCT) domain, which are 79% identical to each other at the sequence level, and are also highly conserved in orthologues of these enzymes in Caenorhabditis elegans, Drosophila, and Xenopus. The CCT domain reflects no similarity to sequences found in other proteins. This domain is known to recognize and interact with a unique peptide (Arg-Phe-Xaa-Val) motif, which is present in OSRT1- and SPAK-activating kinases (with-no-lysine-kinase 1 (WNK1 and WNK4) and also in STE20 kinase family substrates (NKCC1 and NKCC2). It is reported that phosphorylation of a Ser/Thr residue preceding the (Arg-Phe-Xaa-Val) motif results in steric clash, and subsequent dissociation from the CCT domain. Furthermore, patients suffering from Gordon syndrome carry mutated WNK1 and WNK4 genes.
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Species Reactivity:
Human
Mouse
Rat
Canine
Species Reactivity Note:
Demonstrated to react with Human and Mouse.
Predicted to react with Rat and Canine based on 100% sequence homology.
Control:
Hypotonic-stimulated HEK293 cells untreated and lambda phosphatase-treated.
Quality Assurance:
Evaluated by Western Blot in hypotonic-stimulated HEK293 cells untreated and lambda phosphatase-treated.
Western Blot Anlaysis: 0.5 µg/mL of this antibody detected SPAK / OSR1 in 10 µg hypotonic-stimulated HEK293 cells untreated and lambda phosphatase-treated.
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Purification Method:
Affinity Purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q9UEW8
Entrez Gene Number:
NP_037365
Gene Symbol:
STK39
SPAK
Alternate Names:
DCHT
Serine/threonine-protein kinase 39
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
This gene encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that th |