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Anti-phospho-PKD2 Antibody (Ser197/Ser200)
Description:
Anti-phospho-PKD2 Antibody (Ser197/Ser200)
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Specificity:
This antibody recognizes PKD2 phosphorylated at Ser197/Ser200.
Molecular Weight:
~110 kDa observed. An uncharacterized band appears at ~150 kDa in some lysates.
Epitope:
Phosphorylated Ser197/Ser200
Immunogen:
KLH-conjugated linear peptide corresponding to human PKD2 phosphorylated at Ser197/Ser200.
Modifications:
Phosphorylation
Phosphorylation
Background Information:
As members of the calcium-/calmodulin-depedent protein kinase superfamily, there are three known Protein Kinase D (PKD) isoforms, PKD1, PKD2, and PKD3. These serine/threonine kinases are involved in protein-protein and protein-carbohydrate adhesion and are also thought to be ion channel regulators. They are prominent downstream targets of protein kinase C and phospholipase D in various biological systems including mouse. PKDs are activated by various stimuli, including phorbol esters, G-protein-coupled receptors, reactive oxygen species, and diacylglycerol. PKDs regulate protein transport in the trans-Golgi network and are thought to play a role in muscle differentiation, cell motility, migration, and invasion. These isoforms are known to interact with each other. PKD2 is shown to be activated by phorbol esters, both in vivo and in vitro, and also by gastrin via the cholescystokinin/CCK(B) receptor in stably-transfected AGS-B cells. Furthermore, PKD2 is found to be active during the early phase of differentation of C2C12 cells into skeletal muscle cells. It is also reported to enhance TCR-induced NFAT activity in Jurkat T cells.
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Species Reactivity:
Mouse
Human
Rat
Species Reactivity Note:
Demonstrated to react with Mouse.
Predicted to react with Human and Rat based on 100% sequence homology.
Application Notes:
Evaluated by Western Blot in MEF cell lysates untreated and treated with PDBu, Rapamycin, and GO6983.
Western Blot Analysis: A 1:2,000 dilution of this antibody detected PKD2 in 10 µg of MEF cell lysates untreated and treated with PDBu, Rapamycin, and GO6983.
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Control:
MEF cell lysates untreated and PBDu-treated
Quality Assurance:
Evaluated by Western Blot in MEF cell lysates untreated and PBDu-treated.
Western Blot Analysis: A 1:2,000 dilution of this antibody detected PKD2 in 10 µg of MEF cell lysates untreated and PBDu-treated.
Purification Method:
Affinity Purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q13563
Entrez Gene Number:
NP_000288
Gene Symbol:
PC2
PKD4
Pc-2
APKD2
TRPP2
MGC138466
MGC138468
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Alternate Names:
Autosomal dominant polycystic kidney disease type II protein
Polycystic kidney disease 2 protein
Polycystwin
R48321
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
This gene encodes a member of the polycystin protein family. The encoded p |