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EZ-Magna ChIP A/G Chromatin Immunoprecipitation Kit
Description: EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit
Trade Name: Upstate (Millipore)
Qty/Pk: 22 assays
Product Overview: Features & Benefits:
Magnetic bead-based rapid protocol allows performance of ChIP in as little as 1 day
Blend of protein A+G bead blend allows ChIP using a broader range of antibodies than A or G alone
Negative and positive control antibodies and control primer set to simplify validation of experimental procedure
Includes spin columns to make DNA purification easier and more reliable - no more messy phenol-chloroform extractions
Complete kit with all required reagents for reliable and reproducible results
Compatible with ChIPAb+ validated antibody and primer sets
Chromatin Immunoprecipitation (ChIP) is an important technique allowing the analysis of in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powerful application of this technique is to analyze changes in histone modifications that correlate with processes like transcription, mitosis or DNA repair.
Components:
Magnetic Protein A/G Beads
ChIP Dilution Buffer
Low Salt Wash Buffer
High Salt Wash Buffer
LiCl Wash Buffer
TE Buffer
Cell Lysis Buffer
Nuclear Lysis Buffer
ChIP Elution Buffer (w/o Proteinase K)
Proteinase K
RNase A
10X Glycine
10X PBS
Protease Inhibitor Cocktail II
Anti-RNA polymerase II, clone CTD4H8
Normal Mouse IgG
Control Primers
Spin Filters
Collection Tubes
Bind Reagent A
Wash Reagent B
Elution Reagent C |