Ubiquitin-Rhodamine High purity industry standard substrate for deubiquitinylating enzymes (DUBs). Ubiquitin-Rhodamine is prepared by the C-terminal derivatization of ubiquitin with modified rhodamine-110 and has been shown to be a useful and sensitive fluorogenic substrate for wide range of deubiquitinylating enzymes (DUBs), including ubiquitin C-terminal hydrolases (UCHs) and ubiquitin specific proteases (USPs). Ubiquitin-rhodamine is particularly useful for studying deubiquitinylating activity where detection sensitivity or continuous monitoring of activity is essential. The long excitation and emission wavelengths of rhodamine 110 (Excitation 485nm / Emission 520nm) avoid interference often caused at shorter wavelengths by small molecules and tissue culture components. Product Specification Sequence: Accession number: P62988; length: 76 amino acid residues. MW: ~8.9kDa Source: Produced in E. coli and covalently attached to modified rhodamine-110. Formulation: Liquid. In dimethylsulfoxide. Purity: ≥95% (HPLC) Purity Detail: Please note: considerable efforts are taken to ensure that ubiquitin-rhodamine is homogeneous and not contaminated with ubiquitin and ubiquitin deletions, which are common in material obtained from other sources. The chromatogram below demonstrates the homogeneity of Prod. No. BML-SE761. Application: 1. Substrate for deubiquitinylating enzyme activity assays. 2. Identification/confirmation of enzyme deubiquitinylation activity. 3. Investigation of deconjugating enzyme substrate specificity in comparison with alternative UBL substrates. Short Term Storage: -80°C Long Term Storage: -80°C