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RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE
The Ambion® RecoverAll™ Total Nucleic Acid Isolation Kit is for the extraction of total nucleic acid from formalin- or paraformalin-fixed, paraffin-embedded (FFPE) tissues. Sufficient reagents are included for 40 purifications from up to four 20 µm sections, or up to 35 mg of unsectioned core samples each.
Features of the RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE:
• Optimized for isolation of total nucleic acids, including microRNAs, from FFPE tissue
• No overnight Proteinase K digestion required—deparaffinize in the morning and perform qRT-PCR in the afternoon
• Typical yields are >50% of those of unfixed tissue
• Recovered nucleic acids are suitable for real-time RT-PCR, PCR, mutation screening, and microarray analyses
Extraction of Nucleic Acids from Difficult Samples
Archived tissue samples contain valuable information of disease states, but it has traditionally been difficult to isolate nucleic acids from them of a quality suitable for molecular analysis. Standard
Sample Type (General): FFPE & Fixed Samples
Final Product: Genomic DNA,
micro RNA,
Total RNA
Downstream Application: Southern Blotting,
Northern Blotting,
RT-PCR (Endpoint),
PCR,
cDNA Library Construction,
microRNA Analysis,
Microarray Analysis,
Real-Time PCR
High Throughput Compatibility: Manual Protocols,
Not High Throughput-Compatible
Isolation Technology: Spin Column (Glass Fiber Filter)
Product Size: 40 purifications
Yield (up to): 24 µg
Starting Material (Amount): Up to 35 mg of unsectioned core samples,
Up to four 20 µm sections
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
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