|
Screen Quest� FRET No Wash cAMP Assay Kit
Cat # 36379
Storage F/D/L (see storage codes below)
Unit Size 1 plate
Ex (nm) 390
Em (nm) 650
As our kit 36376, Screen Quest� No Wash FRET cAMP Assay Kit provides another convenient assay method for monitoring the activation of adenylyl cyclase in G-protein coupled receptor systems. Compared to other commercial ELISA cAMP assay kits, this homogenous cAMP assay kit does not require a wash step or the acetylation step. The assay is based on the competition for a fixed number of anti-cAMP antibody binding sites between the fluorescent cAMP tracer and non-labeled free cAMP. Free cAMP displaces the fluorescent cAMP tracer from the HRP-cAMP/anti-cAMP antibody complex. The anti-cAMP antibody is labeled with our TR Fluor� Tb while the cAMP tracer contains our bFluor� 650. In the absence of cAMP, bFluor� 650-cAMP conjugate is bound to TR Fluor� Tb-labeled anti-cAMP antibody exclusively to have a strong FRET. However, the unlabeled free cAMP in the test sample competes for the TR Fluor� Tb-labeled anti-cAMP antibody conjugate, therefore inhibits the binding of bFluor� 650-cAMP to anti-cAMP antibody. The bFluor� 650 labeled cAMP tracer only has fluorescence lifetime of nanosecond while TR Fluor� Tb-labeled anti-cAMP antibody-bound fluorescent cAMP tracer has much longer fluorescence lifetime value due to the TR-FRET. The magnitude of FRET is proportional to the concentration of cAMP in a sample. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.
Detailed Information: Protocol References MSDS
Related Products: cAMP GPCR Assays Diagnostic Molecules Cell Signaling
Storage Codes
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
|