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Meter� Cell Viability Assay Kit *Green Fluorescence*
Cat # 22786
Storage F/D/L (see storage codes below)
Unit Size 1 kit
Ex (nm) 495
Em (nm) 515
Our Cell Meter� assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This kit uses the non-fluorescent calcein AM that becomes strongly fluorescent upon entering into live cells. Calcein AM is a hydrophobic compound that easily permeates intact live cells. The hydrolysis of the non-fluorescent calcein AM by intracellular esterases generates the strongly fluorescent hydrophilic calcein that is well-retained in the cell cytoplasm. The esterase activity is proportional to the number of viable cells, and thus directly related to the fluorescence intensity of calcein generated from the esterase-catalyzed hydrolysis of calcein AM. Cells grown in black-walled plates can be stained and quantified in less than two hours. The assay is more robust than the tetrazolium salt or Alarmar Blue�-based assays. It can be readily adapted for high-throughput assays in a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. 營t is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. Using 100 ul of reagents per well in a 96-well format, this kit provides sufficient reagents to perform 200 assays. Using 25 ul of reagents per well in a 384-well format, this kit provides sufficient reagents to perform 800 assays.
Detailed Information: Protocol References MSDS Spectrum
Related Products: Cell Cytotoxicity Viability and Proliferation Cell Viability
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
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