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Cell Explorer� Live Cell Labeling Kit *Green Fluorescence*
Cat # 22607
Storage F/D/L (see storage codes below)
Unit Size 1 kit
Ex (nm) 495
Em (nm) 515
Our Cell Explorer� fluorescence imaging kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to uniformly label live cells in green fluorescence. The kit uses non-fluorescent Calcein Green� that becomes strongly fluorescent upon entering into live cells. Calcein Green� is a hydrophobic compound that easily permeates intact live cells. The hydrolysis of the non-fluorescent Calcein Green� by intracellular esterases generates the strongly fluorescent hydrophilic Calcein Green� fluorophore that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours. It can be readily adapted for high-throughput assays in a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. 營t is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.
Detailed Information: Protocol References MSDS Spectrum
Related Products: Labeling Cells Fuorescence Imaging
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
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