Indo-1, AM

Indo-1, AM
Cat # 21030
Storage F/D/L (see storage codes below)
Unit Size 1 mg
Ex (nm) 346
Em (nm) 475
MW 1009.91
Solvent DMSO
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fura-2 and Indo-1 are most commonly used among the UV-excitable calcium indicators. Fura-2 is excitation-ratioable while Indo-1 is emission-ratioable. Fura-2 is preferred for ratio-imaging microscopy, in which it is more practical to change excitation wavelengths than emission wavelengths. Upon binding Ca2+, Fura-2 exhibits an absorption shift that can be observed by scanning the excitation spectrum between 300 and 400 nm, while monitoring the emission at ~510 nm. In contrast, Indo-1 is the preferred dye for flow cytometry, where it is more practical to use a single laser for excitation (usually the 351�364 nm spectral lines of the argon-ion laser). The emission of Indo-1 shifts to 400 nm (when bound to Ca2+) from 480 nm in Ca2+-free environments. Indo-1 AM is cell-permeable version of Indo-1. 
Detailed Information:   Protocol      References      MSDS      Spectrum
Related Products:     Calcium GPCR Assays     Calcium Channels     pH and Ion Indicators 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)