Fura-2, pentapotassium salt

Fura-2, pentapotassium salt
Cat # 21025
Storage F/D/L (see storage codes below)
Unit Size 1 mg
Ex (nm) 363
Em (nm) 512
MW 832.00
Solvent Water
Among the ratiometric calcium indicators, Fura-2 and Indo-1 are most commonly used. Fura-2 is excitation-ratioable while Indo-1 is emission-ratioable. Fura-2 is preferred for ratio-imaging microscopy, in which it is more practical to change excitation wavelengths than emission wavelengths. Upon binding Ca2+, Fura-2 exhibits an absorption shift that can be observed by scanning the excitation spectrum between 300 and 400 nm, while monitoring the emission at ~510 nm. Fura-2 potassium salt is a cell-impermeable fluorescent probe for detection Ca2+ in a ratiometric mode. 
Detailed Information:   Protocol      References      MSDS      Spectrum
Related Products:     Calcium GPCR Assays     Calcium Channels     pH and Ion Indicators 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)