Fluo-3, pentaammonium salt

Fluo-3, pentaammonium salt
Cat # 21018
Storage F/D/L (see storage codes below)
Unit Size 1 mg
Ex (nm) 506
Em (nm) 526
MW 854.69
Solvent Water
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Rhod-2 are most commonly used among the visible light-excitable calcium indicators. Fluo-3 is essentially nonfluorescent unless bound to Ca2+ and exhibits a quantum yield at saturating Ca2+ of ~0.14 and a Kd for Ca2+ of 390 nM. 
Detailed Information:   Protocol      References      MSDS      Spectrum
Related Products:     Calcium GPCR Assays     Calcium Channels     pH and Ion Indicators 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)