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Acridine orange Cat # 17502 Storage F/D/L (see storage codes below) Unit Size 100 mg Ex (nm) 500 Em (nm) 526 MW 301.82 Solvent Water Acridine orange is a nucleic acid selective fluorescent cationic dye useful for cell cycle determination. It is cell-permeable, and interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, it is very similar spectrally to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm (green). When it associates with RNA, the excitation maximum shifts to 460 nm (blue) and the emission maximum shifts to 650 nm (red). The dye is often used in epifluorescence microscopy. Detailed Information: Protocol References MSDS Spectrum Related Products: DNA Detection Labeling Cells Storage Codes: D = Desiccated L = Avoid light F = Freeze (<-15 癈) R = Refrigerated (2-4 癈) RT = Room temperature (4-50 癈) |