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MMP Red� substrate
Cat # 13521
Storage F/D/L (see storage codes below)
Unit Size 1 mg
Ex (nm) 545
Em (nm) 576
MW ~2000
Solvent DMSO
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix. These enzymes are responsible for the breakdown of connective tissues and are important in bone remodeling, the menstrual cycle and repair of tissue damage. While the exact contribution of MMPs to certain pathological processes is difficult to assess, MMPs appear to play a key role in the development of arthritis as well as in the invasion and metastasis of cancer. MMPs tend to have multiple substrates, with most family members having the ability to degrade different types of collagen along with elastin, gelatin and fibronectin. It is quite difficult to find a substrate that is selective to a single MMP enzyme. This FRET substrate is designed to monitor the general activity of a MMP enzyme. It can also be used to screening MMP inhibitors when a purified MMP enzyme is used. This FRET substrate is based on our TF3/TQ3 FRET pair. Upon MMP hydrolysis the fluorescence of MMP Red� FRET peptide substrate is increased since the TF3/TQ3 FRET pair is separated. The fluorescence increase is proportional to the MMP enzyme activities.
Detailed Information: Protocol References MSDS Spectrum
Related Products: Peptidases and Proteases Miscroplate Readers
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
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