Amplite� Fluorimetric Catalase Assay Kit *Red Fluorescence* Cat # 11306 Storage F/D/L (see storage codes below) Unit Size 1 kit Ex (nm) 571 Em (nm) 585 Catalase is a common antioxidant heme-containing redox enzyme found in nearly all living organisms that are exposed to oxygen. The enzyme is concentrated in the peroxisome subcellular organelles. Hydrogen peroxide is an ROS that is a toxic product of normal aerobic metabolism and pathogenic ROS production involving oxidase and superoxide dismutase reactions. By preventing the excessive buildup of H2O2, catalase allows important cellular processes which produce H2O2 as a by-product to take place safely. The Amplite� Fluorimetric Catalase Assay Kit provides a quick and sensitive method for the measurement of catalase activity. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Catalase reacts with H2O2 to produce water and oxygen (O2). Amplite� Red also reacts with H2O2 to generate a red fluorescent product. Therefore the reduction in fluorescence intensity is proportional to catalase activity. The Amplite� Red substrate used in the assay enables a dual recordable mode. The fluorescent signal can be easily read by either a fluorescence microplate reader at Ex/Em = 540/590 nm or an absorbance microplate reader at ~576 nm. With the Amplite� Fluorimetric Catalase Assay Kit, we have detected as little as 30 mU/mL catalase in a 100 礚 reaction volume. Detailed Information: Protocol References MSDS Spectrum Related Products: Reactive Oxygen Species Storage Codes: D = Desiccated L = Avoid light F = Freeze (<-15 癈) R = Refrigerated (2-4 癈) RT = Room temperature (4-50 癈)
