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Amplite� Fluorimetric Monoamine Oxidase Assay Kit *Red Fluorescence*
Cat # 11303
Storage F/D/L (see storage codes below)
Unit Size 1 kit
Ex (nm) 571
Em (nm) 585
Monoamine oxidases (MAO) are a family of flavin-containing amine oxidoreductases that catalyze the oxidation of monoamines. They are found bound to the outer membrane of mitochondria in numerous tissues including liver, intestinal mucosa, and nerves. In humans there are two types of MAO: MAO-A and MAO-B. MAO-A is particularly important in the metabolism of monoamines ingested in food. MAOs play a major role in the inactivation of neurotransmitters. MAO dysfunction has been associated with depression, schizophrenia, substance abuse, attention deficit disorder, migraines, and irregular sexual maturation. The Amplite� Monoamine Oxidase Assay Kit provides a quick and sensitive method for the measurement of monoamine oxidase and semicarbazide-sensitive amine oxidase (SSAO) activities in blood samples and other biological samples. The kit uses our Amplite� Red substrate which enables a dual recordable mode. The signal can be easily read by either a fluorescence microplate reader at Ex/Em = 540/590 nm or an absorbance microplate reader at ~576 nm. With the Amplite� Monoamine Oxidase Assay Kit, we have detected as little as 10 _U/mL SSAO in a 100 礚 reaction volume. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation.
Detailed Information: Protocol References MSDS Spectrum
Related Products: Diagnostic Molecules
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
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