cAMP-Screen® cAMP Immunoassay System, 96-well The cAMP-Screen® 96-well Cyclic AMP Immunoassay System enables ultrasensitive determination of cyclic AMP (cAMP) levels in cell lysates. This competitive chemiluminescent immunoassay is formatted with maximum flexibility to permit either manual reagent additions or automated high-throughput processing. The cAMP assay utilizes the highly sensitive chemiluminescent CSPD® Substrate with Sapphire-II™ Enhancer that is triggered by an enzyme conjugate composed of cAMP bound to alkaline phosphatase (cAMP-AP). The chemiluminescent substrate/enhancer formulation is a ready-to-use reagent that generates a sustained-glow light emission from 30 minutes after addition. This assay is mainly used in secondary screening and pre-clinical research, where sensitivity and no false positives are essential. • The highest sensitivity of any commercially available cAMP assay • Hours of read time with little or no degradation of the signal • A simple protocol • Useful for a wide range of receptor activation Marker: cAMP Platform: Luminometer (Microplate) Sensitivity: 60 femtomoles cAMP Label or Dye: AP (Alkaline Phosphatase) Product Size: 96 assays Sample Volume: 60 µl Protein Family: GPCR (G-Protein Coupled Receptors) Incubation Time: 60 min Detection Method: Chemiluminescent Research Category: Signal Transduction Sample Type (Specific): Cell Lysates Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures. 1 kit, store at 2 to 8 deg;C
