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PCR Cloning System with Gateway® Technology with pDONR™/Zeo & OmniMAX™2 Competent Cells
Gateway® Technology enables rapid cloning of one or more genes into virtually any protein expression system. Once you have an entry clone, you can recombine your gene of interest into a variety of expression vectors adapted for use in Gateway® Technology. The PCR Cloning System with Gateway® Technology is designed to create an entry clone following PCR with attB-modified custom primers (Figure 1). The PCR product is directionally cloned with efficiencies typically >99%. The PCR Cloning System with Gateway® Technology offers:
■Rapid, efficient, directional cloning of PCR products
■Versatility to use any amplification enzyme
■Cloning without the need for restriction endonucleases or ligase
■Selection of entry clones with kanamycin or Zeocin™
In addition, the pDONR™221 and pDONR™/Zeo vectors have a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use.
Vector Type: pDONR
Cloning Method: Gateway®
Product Size: 20 reactions
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
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