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原装正品|GenMute siRNA Transfection Reagent for Hela Cell

产品编号: SL100568-HELA     查看说明书
产品名称: 原装正品|GenMute siRNA Transfection Reagent for Hela Cell  .0   订购此产品 
供应商: SignaGen
规格: 1.0 ml
目录价: 1980
库存状态: 询盘
CAS编号:
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原装正品|GenMute siRNA Transfection Reagent for Hela Cell
功能:
GenMute™ siRNA Transfection Reagent for Hela Cell is pre-optimized for transfecting siRNA to Hela cells. GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 10 pmoles siRNA or miRNA mimics in 24-well plate.
Refer to the following optimal transfection conditions for maximal silencing on Hela cells. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.
最佳转染条件:
Cell culture medium     DMEM with 4.5 g/L glucose, 10% FBS
Confluency of cell on the day of transfection  ~50%
Optimal siRNA concentration    20 nM
Diluent for siRNA and GenMute? Reagent   1xGenMute? Transfection Buffer
Incubation Time to Form GenMute?/siRNA Complex  ~15 minutes at RT
Maximal Silencing Efficiency     48 hours
Transfection Results:
Reporter Gene   Renilla Luciferase 
Silencing Efficiency (% ) 90%
储存条件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

保存条件: 4°C
说明书地址: 点击查看详细
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