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AMC Arachidonoyl Amide
Cayman Chemical Item Number 10005098
Description
AMC arachidonoyl amide (AMC-AA) is one of several fatty acid amides which can be used to measure fatty acid amide hydrolase (FAAH) activity.1↗ FAAH is a relatively unselective enzyme in that it accepts a variety of amide head groups other than the ethanolamine of its nominal endogenous substrate anandamide (AEA).2↗ Exposure of AMC-AA to FAAH activity results in the release of the fluorescent aminomethyl coumarin that absorbs at 360 nm and emits at 465 nm. This allows the fast and convenient measurement of FAAH activity using a simple cuvette or microplate fluorometer.
1 Cravatt, B.F., Giang, D.K., Mayfield, S.P., et al. Molecular characterization of an enzyme that degrades neuromodulatory fatty-acid amides. Nature 384 83-87 (1996).
2 Patricelli, M.P., and Cravatt, B.F. Characterization and manipulation of the acyl chain selectivity of fatty acid amide hydrolase. Biochemistry 40 6107-6115 (2001).
Synonyms AMC-AA
7-amino-4-methyl Coumarin-Arachidonamide
Formal Name 7-amino-4-methyl-2H-1-benzopyran-2-one-5Z,8Z,11Z,14Z-eicosatetraenamide
CAS Number 862913-13-1
Molecular Formula C30H39NO3
Formula Weight 461.6
Formulation A crystalline solid
Purity ≥98%
λmax 229, 328 nm
Stability 2 years
Storage -20°C
Shipping Room temperature in continental US; may vary elsewhere
SMILES CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(=O)Nc1ccc2c(C)cc(=O)oc2c1
InCHI Code 1S/C30H39NO3/c1-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-20-29(32)31-26-21-22-27-25(2)23-30(33)34-28(27)24-26/h7-8,10-11,13-14,16-17,21-24H,3-6,9,12,15,18-20H2,1-2H3,(H,31,32)/b8-7-,11-10-,14-13-,17-16-
InCHI Key IQPBUDMTXHWSHB-ZKWNWVNESA-N |