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CRTH2/DP2 Receptor (N-Term) Blocking Peptide
Cayman Chemical Item Number 10004884
Description
Peptide sequence: CRTH2/DP2 protein amino acids 2-21 · To be used in conjunction with Cayman’s CRTH2/DP2 Receptor (N-Term) polyclonal antibody (Item No. 10004886) to block protein-antibody complex formation during immunochemical analysis of CRTH2/DP2 · Prostaglandin D2 (PGD2) elicits its biological function through interaction with two distinct G protein-coupled receptors, DP1 and CRTH2/DP2. CRTH2 primarily couples to Gi or Gq subunits to mobilize Ca2+, induce cell migration, and up-regulate adhesion molecules.1↗ CRTH2 mRNA has been detected in various tissues including liver, lung, kidney, brain, heart, thymus, and spleen and in various cell lineages including both hematopoietic and non-hematopoietic cell lines.2↗ Human CRTH2 is 395 amino acids in length with an estimated molecular weight of 43 kDa. Cayman’s CRTH2/DP2 receptor (N-Term) polyclonal antibody can be used for western blot and immunohistochemical analysis of CRTH2 on samples of human, bovine, murine, and rat origin. It detects both unglycosylated and glycosylated protein at sizes ranging from 35-40 kDa to 50-70 kDa, as reported by Nagata et al., in 1999.3↗
1 Nagata, K., and Hirai, H. The second PGD2 receptor CRTH2: Structure, properties, and functions in leukocytes. Prostaglandins Leukot Essent Fatty Acids 69 169-177 (2003).
2 Abe, H., Takeshita, T., Nagata, K., et al. Molecular cloning, chromosome mapping and characterization of the mouse CRTH2 gene, a putative member of the leukocyte chemoattractant receptor family. Gene 227 71-77 (1999).
3 Nagata, K., Tanaka, K., Ogawa, K., et al. Selective expression of a novel surface molecule by human Th2 cells in vivo. J Immunol 162 1278-1286 (1999).
Synonyms DP2 Receptor
Chemoattractant-receptor homologous molecule expressed on Th2 cells
G protein-coupled receptor 44
Prostaglandin D2 receptor 2
Formal Name
Formulation 200 µg peptide in 200 µl TBS, pH 7.4, containing 0.1% BSA and 0.02% sodium azide
Stability 2 years
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere |