PGN-SAndi ultrapure, insoluble

PGN-SAndi ultrapure
Insoluble Peptidoglycan from Staphylococcus aureus
Peptidoglycan (PDN) is a major surface component of Gram-positive bacteria. It is embedded in a relatively thick cell wall and is usually covalently attached to other polymers, such as lipoproteins and LTAs. PGN is known to be a potent activator of NF-κB and TNF-α through TLR2 [1].However, other pattern recognition proteins have been reported to mediate the immunostimulatory activity of PGN [2, 3, 4]. This discrepancy is correlated to the method of purification. According to Travassos et al., the presence of these polymers in most preparations of PGN is responsible for the TLR2-dependent activity of PGN [2].
PGN-SAndi from S. aureus is an insoluble preparation of PGNs purified by detergent lysis and hydrolysis under basic conditions to eliminate lipophilic constituents.This PGN preparation has lost its ability to activate TLR2-transfected HEK293 cells but still activate NOD2-transfected cells.
Specificity: NOD1/NOD2 agonist
Working Concentration: 1 - 5 µg/ml
Endotoxin level:  EndoFit™ (<0.001 EU/μg)
Resuspension: 200 μg/ml in water. Note: PGN-SAndi ultrapure is insoluble. The suspension appears hazy.