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CL264 Rhodamine
Rhodamine labeled CL264
CL264 is a novel 9-benzyl-8 hydroxyadenine derivative containing a glycine on the benzyl group (in para). Similarly to SM360320, CL264 induces the activation of NF-κB and the secretion of IFN-α in TLR7-expressing cells [1]. CL264 is a TLR7-specific ligand, it does not stimulate TLR8 even at high concentrations (> 10 µg/ml). In TLR7-transfected HEK293 cells, CL264 triggers NF-κB activation at a concentration of 0.1 µM which is 5-10 times less than imiquimod. CL264 is labeled with Rhodamine on the acid group of the glycine via a HEX spacer. CL264 Rhodamine can be used confocal laser-scanning microscopy or flow cytometry.
Specificity: human/mouse TLR7 agonist
Working concentration: 1 -10 μg/ml
Endotoxin level: EndoFit™ (<0.001 EU/μg)
Solubility: 10 mg/ml in DMSO
Spectral Properties of Rhodamine
Excitation λ max: 552 nm
Emission λ max: 570 nm |