PGN-ECndss ultrapure, soluble

PGN-ECndss ultrapure
Soluble sonicated peptidoglycan from E. coli K12
Gram positive peptidoglycan (PGN) is usually bound to other polymers such as teichoic acids and carbohydrates, while Gram negative PGN is commonly attached covalently to a single type of lipoprotein. According to Travassos et al., the presence of these polymers in most preparations of PGNs is responsible for the TLR2-dependent activity of PGN [1]. PGNs purified by detergent lysis and hydrolysis under basic conditions to eliminate lipophilic constituents lose their ability to activate TLR2-transfected HEK293 cells but still activate NOD2-transfected cells. Insoluble PGN from E. coli also activates NOD1-transfected cells.
Soluble PGN from E. coli K12 is obtained after sonication of insoluble PGNs. At the working concentrations, it activates  HEK-Blue™ NOD1 and HEK-Blue™ NOD2 cells but not HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.
Specificity: NOD1/NOD2 agonist
Working Concentration: 1 - 5 µg/ml
Endotoxin level:  EndoFit™ (<0.001 EU/μg)
PGN-ECndss ultrapure is provided as a solution at 1 mg/ml