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Anti-Potassium Channel Kv1.3, clone L23/27
Species Reactivity Key Applications Host Format Antibody Type
R WB, ICC Mouse Purified Monoclonal Antibody
Description:
Anti-Potassium Channel Kv1.3 Antibody, clone L23/27
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Specificity:
This antibody recognizes Kv1.3 at the C-terminus.
Molecular Weight:
~58 kDa observed. The molecular weight band at ~ 150 kDa could be the heterotetramer of Kv1.3. The ~ 23kDa band could be a truncate fragment of kv1.3 truncate fragment. (Tu, L., et al. (1996) J. Biol. Chem. 271(31):18904−18911.)
Epitope:
C-terminus
Immunogen:
Linear peptide corresponding to rat Kv1.3 at the C-terminus.
Clone:
L23/27
Isotype:
IgG2a
Background Information:
Potassium channel Kv1.3 is a voltage gated channel most commonly found in the plasma membrane, and recently also found in the inner mitochondrial membrane. It is thought that Kv1.3 is instrumental in the apoptosis signaling pathway. During the onset of apoptosis, Bax binds to Kv1.3 and hinder its function, this action causes hyperpolarization. This increases the production of reactive oxygen species and cytochrome c. Kv1.3 may also be a member of the pathway in cells outside of just lymphocytes.
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Species Reactivity:
Rat
Species Reactivity Note:
Demonstrated to react with rat.
Application Notes:
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. (Doczi, M., et al. (2008). Am J Physiol Regul Integr Comp Physiol 295:R733-R740.)
Control:
Rat brain membrane prep tissue lysate
Quality Assurance:
Evaluated by Western Blot in rat brain membrane prep tissue lysate.
Western Blot Analysis: 5 µg/mL of this antibody detected Kv1.3 on 10 µg of rat brain membrane prep tissue lysate.
Purification Method:
Protein G
Presentation:
Purified mouse monoclonal IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P15384
Gene Symbol:
KCNA3
HLK3
HGK5
KV1.3
HuKIII
HPCN3
Kv1.3
MK3
HUKIII
PCN3
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Alternate Names:
potassium voltage-gated channel, shaker-related subfamily, member 3
potassium channel 3
potassium voltage-gated channel subfamily A member 3
voltage-gated potassium channel protein Kv1.3
Voltage-gated potassium channel subunit Kv1.3
Voltage-gated K(+) channel HuKIII
type n potassium channel
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
UniProt Summary:
FUNCTION: Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
SUBUNIT STRUCTURE: Heterotetramer of potassium channel proteins (By similarity). Binds PDZ domains of DLG1, DLG2 and DLG4.
SUBCELLULAR LOCATION: Memb |