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Anti-Pick1, clone L20/8
Species Reactivity Key Applications Host Format Antibody Type
R WB Mouse Purified Monoclonal Antibody
Description:
Anti-Pick1 Antibody, clone L20/8
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Molecular Weight:
~ 47 kDa observed. The ~ 90 kDa band could be the homodimer.
Immunogen:
Recombinant protein corresponding to rat Pick1.
Clone:
L20/8
Isotype:
IgG2aκ
Background Information:
Pick1 is thought to be involved in the organization and localization of a number of membrane proteins (such as AMPA receptors) containing the PDZ recognition sequence. It has been shown that Pick1 binds phosphoinositide lipids using the Bin/amphiphysin/Rvs (BAR) domain. This step is critical for the targeting function of Pick1, its ability to move AMPA receptors to membrane surfaces and the induction of long-term signal depression.
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Species Reactivity:
Rat
Species Reactivity Note:
Demonstrated to react with rat.
Control:
Rat brain membrane tissue lysate
Quality Assurance:
Evaluated by Western Blot in rat brain membrane tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Pick1 on 10 µg of rat brain membrane tissue lysate.
Purification Method:
Protein G
Presentation:
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q9EP80
Entrez Gene Number:
NP_445912
Gene Symbol:
PICK1
Pick1
PRKCABP
PICK
Alternate Names:
protein interacting with PRKCA 1
Protein kinase C-alpha-binding protein
protein interacting with PRKCA
protein kinase C, alpha binding protein
PRKCA-binding protein
Protein interacting with C kinase 1
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
PDZ domain containing protein; interacts with C-terminal domain of many central nervous system receptor proteins; may have an important role in signal transduction during synaptic transmission [RGD].
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. May be regulated upon PRKCA activation. May regulate ACCN3/ACCN2 channel.
SUBUNIT STRUCTURE: Monomer and homodimer. Interacts with CXADR. Interacts presynaptically with the glutamate receptors GRIA2, GRIA3, GRIK3, isoform 3 of GRIA4, isoform A of GRM4, GRM7 and GRM8; with NAPA and NAPB; and with BTG2. The interaction with NAPA and NAPB disrupts the interaction with GRIA2, conducting to the internalization of GRIA2. Interacts with PRKCA; with the amine transporters SLC6A2 and SLC6A3; with the channels ACCN1 and ACCN2; with the GTP-binding proteins ARF1 and ARF3; with the ephrin receptor tyrosine kinases EPHA7, EPHB1 and EPHB2; with ERBB2 and through its PDZ domain with the C-terminal tail of PRLHR By similarity. Interacts with UNC5A. Interacts (via AH domai |