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Anti-pan-MAGUK, clone K28/86
Species Reactivity Key Applications Host Format Antibody Type
R, H, M IHC, WB, IP Mouse Purified Monoclonal Antibody
Description:
Anti-pan-MAGUK Antibody, clone K28/86
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Specificity:
This antibody exhibits cross reactivity against recombinant Chapsyn-110, SAP97, and SAP102. (UC Davis/NIH NeuroMab Facility, http://neuromab.ucdavis.edu)
Molecular Weight:
~ 85-110 kDa observed
Immunogen:
Recombinant protein corresponding to a fusion domain of human PSD95/SAP90 proteins.
Clone:
K28/86
Isotype:
IgG1λ
Background Information:
The MAGUKs are membrane-associated guanylate kinase related (MAGUK) proteins that are defined by their inclusion of PDZ, SH3 and GUK domains. The GUK domain that they have is structurally very similar to that of the guanylate kinases, which converts ATP into ADP. However, it is known to be catalytically inactive as the P-Loop which normally binds ATP is absent. It is thought that the MAGUKs have adopted the GUK domain for their own purposes, primarily based on its ability to form protein-protein interactions with cytoskeleton proteins, microtubule/actin based machinery and molecules involved in signal transduction. In addition many of the MAGUK proteins also contain regions homologous of CaMKII, WW and L27 domains. Some of the members of the MAGUK family of proteins include DLG4 (Disks large homolog 4), DLG2, PSD95, MPP1 and other cytoskeleton-interacting proteins.
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Species Reactivity:
Rat
Human
Mouse
Species Reactivity Note:
Demonstrated to react with rat. Predicted to react with human based on immunogen design. Predicted to react with mouse based on 100% sequence homology.
Application Notes:
Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected the MAGUK family of proteins in rat cerebellum and rat hypothalamus tissues.
Immunoprecipitation Analysis: A previous lot of this antibody was successfully used by an independent laboratory in IP. (Rasband, M., et al. (2002). The Journal of Cell Biology. 159(4):663–672.)
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Control:
Rat brain tissue lysate
Quality Assurance:
Evaluated by Western Blot in rat brain tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected members of the MAGUK protein family on 10 µg of rat brain tissue lysate.
Purification Method:
Protein G
Presentation:
Purified mouse monoclonal IgG1λ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P78352
Entrez Gene Number:
NP_001356
Gene Symbol:
DLG4
SAP90
PSD95
SAP-90
PSD-95
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Alternate Names:
discs, large homolog 4 (Drosophila)
discs large homolog 4
disks large homolog 4
Tax interaction protein 15
post-synaptic density protein 95
Postsynaptic density protein 95
Synapse-associated protein 90
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunohistochemistry
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