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Anti-GluR2, clone L21/32
Species Reactivity Key Applications Host Format Antibody Type
R, M, H WB, IHC Mouse Purified Monoclonal Antibody
Description:
Anti-GluR2 Antibody, clone L21/32
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Molecular Weight:
~ 96 kDa observed
Immunogen:
Recombinant protein corresponding to human GluR2.
Clone:
L21/32
Isotype:
IgG1κ
Background Information:
Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. Although subunit composition of AMPA receptors varies, they must contain at least one edited GluR2 subunit to be calcium impermeable. The critical residue controlling calcium permeability is in the pore loop region. In GluR1, GluR3, and GluR4, this position is occupied by a Gln residue. In GluR2, it is occupied by an Arg residue. It has been shown experimentally that the presence of Arg in this position blocks Ca2+ ion permeability, while a Gln does not. Relative calcium permeability in AMPA receptor channels may be significant in pathological neurotoxic damage and long term changes in nervous system responses.
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Species Reactivity:
Rat
Mouse
Human
Species Reactivity Note:
Demonstrated to react with rat. Predicted to react with human based on immunogen design. Predicted to react with mouse based on 100% sequence homology.
Application Notes:
Immunohistochemistry Analysis: 1:300 dilution of this antibody from a previous lot detected GluR2 in rat cerebellum and hippocampus tissue.
Control:
Rat brain membrane tissue lysate
Quality Assurance:
Evaluated by Western Blot in rat brain membrane tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected GluR2 on 10 µg of rat brain membrane tissue lysate.
Purification Method:
Protein G
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P42262
Entrez Gene Number:
NP_000817
Gene Symbol:
HBGR2
GLUR2
GLURB
GluR-K2
GluR-B
GluR-2
gluR-B
GRIA2
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Alternate Names:
glutamate receptor, ionotropic, AMPA 2
glutamate receptor 2
Glutamate receptor ionotropic, AMPA 2
AMPA-selective glutamate receptor 2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry
Entrez Gene Summary:
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole pr |