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Anti-Caspr, clone K65/35
Species Reactivity Key Applications Host Format Antibody Type
R IHC, WB Mouse Purified Monoclonal Antibody
Description:
Anti-Caspr Antibody, clone K65/35
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Molecular Weight:
~ 220 kDa observed
Immunogen:
Recombinant protein corresponding to rat Caspr.
Clone:
K65/35
Isotype:
IgG1κ
Background Information:
Contactin-associated protein 1(Caspr) is a single-pass type I membrane protein belonging to the neurexin family and is comprised of one F5/8 type C domain, one fibrinogen C-terminal domain, two EGF-like domains, and four laminin G-like domains. Caspr is well documented for its interaction with contactin, an interaction that is crucial to the transport of Caspr to the plasma membrane, the mylination of glial cells, and the generation of axoglial junctions. Studies also suggest that Caspr may play a regulatory role in the cell surface expression of contactin and its targeting to different axonal domains. Expression of Caspr is largely observed in the brain, mainly in CNS myelinated nerve fibers within paranodal axoglial junctions.
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Species Reactivity:
Rat
Species Reactivity Note:
Demonstrated to react with rat. Predicted to react with mouse based on 100% sequence homology.
Application Notes:
Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected Caspr in rat cerebellum and rat hippocampus tissues.
Control:
Rat brain membrane tissue lysate
Quality Assurance:
Evaluated by Western Blot in rat brain membrane tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Caspr on 10 µg of rat brain membrane tissue lysate.
Purification Method:
Protein G
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P97846
Entrez Gene Number:
NP_114450
Gene Symbol:
CNTNAP
Caspr1
caspr1
P190
p190
NRXN4
CASPR
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Alternate Names:
contactin associated protein 1
neurexin 4
neurexin-4
contactin-associated protein 1
Neurexin IV
Neurexin-4
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunohistochemistry
Western Blotting
Entrez Gene Summary:
Forms a complex with contactin; may mediate communication between glial cells and neurons during development [RGD].
UniProt Summary:
FUNCTION: Seems to play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the paranodal region of the axo-glial junction. In association with contactin may have a role in the signaling between axons and myelinating glial cells.
SUBUNIT STRUCTURE: Interacts with contactin in cis form.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (Potential).
TISSUE SPECIFICTY: Predominantly expressed in brain. In myelinated nerve fibers of the CNS predominantly found in paranodal axoglial junctions. In un |